HANDBOOK FOR BIO-CHEMICAL LABORATORY. 37 



of equal parts alcohol and water, which dissolves the diastatic 

 ferment, a little tyrosin, and some salts, leaving some albumin 

 undissolved. The liquid is dialyzed, concentrated in vacuo, 

 and precipitated by absolute alcohol. The precipitate thus 

 obtained possesses feeble proteoly tic properties, due to remain- 

 ing traces of the proteolytic ferment, but has intense diastatic 

 properties. (Danielewslci.) 



The Fat-decomposing Ferment of the Pancreas 



(Steapsin). 



Preparation. A perfectly fresh pancreas is crushed in a 

 mortar with glass powder and mixed with a solution com- 

 posed of 90 c.c. glycerin and 1 c.c. of a \% solution of K"a 2 C0 3 , 

 using 30 c.c. of the glycerin solution for every 3 grins, of the 

 pancreas. This is allowed to digest for not longer than four 

 to five days and then removed from the mass of the pancreas. 

 This extract has the property of decomposing neutral fats. 

 ( Griitzner. ) 



Leucin, C a H 13 K0 2 . 

 a. AMIDO-ISOBUTYL ACETIC ACID, 

 (CH 3 ) 2 CH.CH 2 .CH(NH S ).COOH. 



Tyrosin, C 9 H n N0 3 . 



p. OXYPHENYL-AMIDO PfiOPIONIC ACID, 

 J00H 



CH a .CH(NH 2 )COOH. 



Preparation. 1. These two bodies may be prepared in 

 large quantities by boiling albuminous bodies or albuminoids 

 with dilute mineral acids. Two parts hoof-shavings (J to 1 

 kilo in weight) are boiled for 24 hours with 5 parts concen- 

 trated sulphuric acid and 13 parts water, adding water from 

 time to time to replace that which has evaporated. After 



