HANDBOOK FOR BIO-CHEMICAL 

 LABORATORY. 



Olycogen, C 6 H 10 5 . 



Preparation. 1. Kill a large well-fed rabbit by cutting its 

 throat, open the abdomen immediately and remove the liver. 

 After weighing, cut it up in rather large pieces and quickly 

 throw them in boiling water (about 400 c.c. to 100 grms. liver), 

 and let boil for half an hour. Then remove the pieces and 

 grind them up finely in a mortar, return to the boiling water 

 and add caustic potash solution (3-4 grms. KOH to 100 grms. 

 liver). Now warm on the water-bath, and allow it to concen- 

 trate until you have 200 c.c. of liquid for every 100 grms. liver. 

 If a scum forms on the surface, place the liquid in a beaker, 

 covering it with a watch-glass, and heat it until all has dis- 

 solved, then put aside to cool. Neutralize with HC1 and pre- 

 cipitate the albuminous bodies by the alternate addition of HC1 

 and a solution of potassio-mercuric iodide * ^Brilcke reagent) 

 in small portions. The addition of potassiovmercuric iodide 

 must be continued until no further precipitate occurs. If 

 the liquid at last remains milky, nearly neutralize with 

 caustic soda, and then treat with HC1 again. Filter off the 

 precipitate of albuminous bodies through thick filter-paper, 

 and wash by removing the precipitate from the filter by means 

 of a spatula and place it with water containing HC1 and 



* This solution is prepared by saturating a boiling, not too concen- 

 trated (10*) solution of potassium iodide with pure mercuric iodide and 

 filtering after cooling. 



