90 HANDBOOK FOR BlO-CHEMlOAL LABORATORY. 



PIRIA'S Test (Ty rosin}. Dissolve the substance in con- 

 centrated sulphuric acid and allow to stand for -J- an hour. 

 Dilute with water and neutralize the solution with BaC0 3 and 

 filter. On the addition of acid-free ferric chloride to the clear 

 filtrate a violet color is produced in the presence of tyrosin. 

 The reaction is impeded by the presence of free acid. 



POHI/S Test (Globulins). He suggests to saturate the solu- 

 tion to one-half with ammonium sulphate, which precipitates 

 the globulins. Filter and wash with a one half saturated 

 solution of ammonium sulphate. 



PROTEIDS. See Adamkiewicz, Biuret, Brilcke, Frohde, 

 Furfur ol, Grigg, Liebermann, Mandel, Millon, Mulder, Mi- 

 cliailou, Petri, Piotrowski, Reichl, Schultze, Xantho-proteic. 



Pus. See Donne. 



KA ABE'S Test (Albumin). Place 1 c.c. of the liquid to be 

 tested in a test-tube and add a small piece of trichloracetic 

 acid. In the presence of albumin a white zone or ring will 

 be formed. The ring produced by uric acid is diffused and 

 not sharply defined. 



RABUTEAU'S Test (HCl in Contents of Stomach). Add 

 the filtered contents of the stomach to a solution containing 

 50 c.c. starch mucilage, 1 grm. potassium iodate, and 0.5 grm. 

 potassium iodide, In the presence of free HCl it will become 

 blue. 



REES'S Test (Albumin). An alcoholic solution of tannic 

 acid precipitates small amounts of albumin. 



REICHL'S Test (Proteids). Add 2-3 drops of an alcoholic 

 solution of benzaldehyde to the proteid solution, and then 

 considerable sulphuric acid which has previously been diluted 



