92 HANDBOOK FOR BIO-CHEMICAL LABORATORY. 



obtain the percentage of glucose or grammes per 100 c.c. of 

 urine. 



ROBERT'S Test (Albumin). Allow the urine to flow on the 

 surface of a saturated common salt solution containing 5$ 

 HC1 of specific gravity 1.052. In the presence of albumin a 

 white ring or zone will form between the two liquids. 



He also suggests a mixture of 1 part strong nitric acid and 

 5 parts saturated magnesium sulphate solution. It is to be 

 applied as above, 



ROSENBACH'S Modification of GMELIN'S Test (Bile Pig- 

 ments). Filter the liquid through a very small filter. When 

 all liquid has passed through, apply to the inside of the filter 

 a drop of nitric acid which contains only very little nitrous 

 acid. A pale yellow spot will be formed, which is surrounded 

 by colored rings which are yellowish red, violet, blue, and 

 green. 



ROSENBACH'S Test (Indigo Red or Indirubiri). On boiling 

 the liquid with nitric acid indigo blue is formed from the 

 indigo red. 



ROSIN'S Test (Indigo Red or Indirubin). Make the liquid 

 alkaline with sodium carbonate and extract with ether, which 

 is colored red by the indigo red. 



RUBNER'S Test (Carbon Monoxide in Blood). Shake the 

 blood for one minute with 4-5 volumes lead acetate solution. 

 If the blood contains CO, it will retain its bright red color, 

 while if it does not it will turn chocolate brown. 



RUBBER'S Test (Glucose). Treat the liquid with an excess 

 of lead acetate, filter, and add ammonia to the filtrate until 

 no further precipitate is produced. Warm gently, when the 



