CONTENTS. 



PAGE 



PARTI. PROTOZOA .. .. .. .. .. .. .. 1 



A. GENERAL SUEVEY .. .. ' 1 



1. Introductory : Upon the Organization of the Protozoa . . . . . . 1 



2. General Remarks upon the Technique of the Examination of Protozoa . . 4 

 Examination of the living object . . . . . . . . . . 4 



Fixing mixtures . . . . . . . . . . . . . . 7 



Cover-glass preparations . . . . . . . . . . . - 9 



Preparation of sections . . . . . . . . . . 10 



Staining .. .. .. .. .. .. .. ..11 



B. SPECIAL .. .. .. .. .. .. .. ..14 



Class I. : RHIZOPODA . . .. .. . . .. .. ..14 



Order : Amcebina . . . . . . . . . . . . 14 



(a) Free-living Amoebae ; Methods of cultivation . . . . . . 14 



(b) Parasitic Amcebse . . . . . . . . . . 18 



Class II. : NEOSPORIDIA . . . . . . . . . . 22 



Order 1. Microsporidia . . . . . . . . . . 23 



Order 2. Myxosporidia . . . . . . . . . . 26 



(a) Myxidium lieber kilhni .. .. -r*^.. .. .. 26 



(b) Sphceromyxa iabrazesi (formation of cnidospores) . . . . 28 



(c) Myxobolida .. .. .. .. .. ..29 



Order 3. Actinomyxidia . . . . . . . . . . 32 



Order 4. Sarcosporidia ... .. .. .. .. ..33 



Class III. : FLAGELLATA . . . . . . . . . . 36 



Order 1. Protomonadina (Bodo) . . . . . . . . 38 



Order 2. Polymastigina . . . . . . . . . . 39 



(a) Trichomonas and Trichomastix . . . . . . ... 40 



(b) Lctmblia .. .. .. .. .. .. ..41 



(c) Costia .-. . . . . .. .. . . ..41 



Order 3. Binucleata (Heemoflagellates and Hsemosporides) . . . . 42 



A. General Directions for Obtaining and Examining Material . . 42 



1. Methods of obtaining material . . . . . . 42 



2. Inoculation . . . . . . . . . . 44 



3. Cultivation outside the body . . . . . . 46 



4. Transmission by blood-sucking animals .. .. .. 48 



Stinging Flies . , . . . . . . 51 



5. Examination of blood . . . . . . . . 54 



