96 



COAL-TAR COLORS USED IN FOOD PRODUCTS. 



Experimental data by Chlopin Continued. 



No. 2. 

 [1 gram =149 ing =104. 3 grains.] 



2. MEYER (/. Amer. Chem. Soc., 1907, v. 29, p. 900}: Dog receiving 113 milligrams 



per kilogram body weight; that is, 79 grains per 100 pounds body weight. Result, 

 diarrhea, which continued throughout 17 days, although only 3 doses of the 

 same size were given in 6 days; thereafter the dose was increased geometrically 

 the diarrhea continued; the coloring matter was discharged in the urine anc 

 the feces; there was no abnormal condition revealed by the autopsy. 



DOUBTFUL. 



1. WINOGRADOW (Zts. Ndhr. Genussm., 1903, v. 6, p. 589} says it noticeably retards 

 digestive action; is not indifferent. 



G. T. 88. 



Trade names. Diphenylamin Orange; Orange IV', Tropaeolin OO; 

 Orange M; Fast Yellow; Orange G S; New Yellow; Orange N; Acid 

 Yellow D. 



Scientific name. Sodium salt of para-sulphobenzene-azo-diphenyl- 

 amin. 



Discovered. 1876. 



Shade. Orange Yellow. Not offered. 



FAVORABLE. 



1. WEYL (p. 115}: "Nonpoisonous * * * Diphenylamin Orange * * * 

 2 * * * Diphenylamin Orange is * * * nonpoisonous." (p. 132.} 



3. "For instance, the poisonous Metanil Yellow corresponds to the nonpoisonous 



Diphenylamin Orange." (p. 148.) 



4. The experiments on which Weyl based the conclusions above may be summarized 



as follows: A. A dog received 183 milligrams per kilogram body weight, or 

 128 grains per 100 pounds body weight. The urine was rendered black, and 

 contained traces of phen.ol and abundant albumen; this condition lasted for 

 72 hours, at the end of which 110 milligrams per kilogram body weight, or 

 77 grains per 100 pounds body weight, were administered; urine became 

 colorless and albumen diminished. Five days afterwards a total of 10 grams, 

 or 366 milligrams per kilogram body weight, or 256 grains per 100 pounds 

 body weight, were administered; the urine became abundant, was strongly 

 black, alkaline, contained albumen. The loss of weight was 1 kilogram, or 

 about 3 per cent. B. A dog received 308 milligrams per kilogram, or 216 grains 

 per 100 pounds body weight, as the initial dose; albuminuria did not result 

 until after repeated dosing same as initial dose, and administered for 2 weeks. 



5. Weyl's conclusions are as follows: "According to the above investigations, 



Diphenylamin Orange causes albuminuria, . but further disturbances did not 

 appear during the several weeks' observations on the animals used." 



