AN ANALYSIS OF THE EFFECT OF SELECTION. 17 
TaBLE 14.—868, Inbred Minus Line. 
Generation. n M o 
re ie cere ier 74 | 4.432+0.070 | 0.888+0.049 
Wgiera = chemteurs 109 | 4.688+ .053 -820+ .037 
Bye sence 193 | 4.104+ .042 .834+ .029 
Wg iwaiers estetere 68 | 3.765+ .063 -768+ .044 
LY Reena ns 84 | 4.286+ .053 -716+ .037 
eRe codas 22 | 4.228= .106 .736+ .075 
550 
REVERSED SELECTION. 
le Oneesats 112 | 4.732+0.055 | 0.856+0.038 
1 Dares BERG 225 | 4.862+ .039 -866+ .027 
337 
CROSSBRED MINUS SERIES. 
The following cultures furnished the material for this series: 
Cultures 920, 1063, 1073, 1082 of the 900 minus line. 
Cultures 935, 936, 1047 of the 868 minus line. 
Culture 942, made up by mating together two 4-bristled Dichets 
from 912, which in turn was the result of mating a sepia, spineless, 
kidney, sooty, rough male to a female from a daughter culture of 
869 (see pedigree of 900 line). 
: are. LNA 
Culture 949, made up by mating a female of the constitution —-~———* 
p?sseTo 
(from the cultures of Mr. J. W. Gowen) to a male from culture 916 
(see pedigree of 1002 line). 
All the cultures in this series traced to 868, and the ‘‘generation”’ 
given is the greatest number of generations from 868, which is thus 
the standard for this line, just as 864 was for the crossbred plus series. 
Table 15 and figure 8 give the results for the series. Here again, 
the effectiveness of selection is suggested, but is doubtful. The means, 
however, are lower than in any other series except the 868 line, and 
that line entered very largely into the make-up of this one. 
Speck Minus or 1331 Line. 
In connection with certain experiments to be described below it 
became desirable to have a minus line that should be recessive for some 
second chromosome character. Accordingly culture 1331 was made 
up by mating a 4 female from 1168, Fs, of the crossbred minus series, 
to a speck male.’ The line was then inbred, in pairs, brother to sister, 
minus selected, and gradually made homozygous for speck, sepia, and 
rough. 
