22 ANAPHYLAXIS AND ANTI-ANAPHYLAXIS 



of 1 00° and even of 120° C. Very often we have 

 sensitised guinea-pigs with milk that has been steri- 

 lised in the autoclave at 120° C. for a quarter of an 

 hour; the result has been quite as good as with milk 

 heated to 100° C. or with unboiled milk. 



When heated to higher temperatures — 130° to 

 140° C. — the milk sensitises less and less satisfac- 

 torily. We have noted that its sensitising power 

 advances, from the point of view of resistance to 

 the temperature, pari passu with its toxic power. 

 As we shall see later, the two functions, the sensitising 

 and the toxic, are resistant to 120° C; beyond that 

 temperature they decline, and disappear completely 

 when the temperature approaches 135° to 140° C. 



Let us observe by the way that this parallelism is 

 not absolute: it does not hold good for sera. The 

 sensibiligen of the latter is thermostable ; their toxic 

 power is, on the contrary, thermolabile. Already 

 diminished at 70° C, this power, in the case of sera, 

 declines progressively with the rise of temperature. 

 At 100° C. the toxic power no longer exists, whilst the 

 serum sensitises fully. 



We have repeatedly attempted to sensitise guinea- 

 pigs with milk by way of the mouth or rectum. We 

 have introduced into the mouths of a large number 

 of guinea-pigs from 3 c.c, to 7 c.c. of unboiled milk; 

 then after varying intervals (16, 18, 23, 30, 44 days) 

 we have tested them by intracerebral injections 

 (0-25 c.c). Never, in the course of our experiments, 

 have we noticed the slightest symptom of anaphylaxis. 



In the same way the guinea-pigs which we attempted 

 to sensitise per rectum always proved to be resistant 

 to the intracerebral injection of milk. In order to 

 favour the absorption of milk per rectum we first 

 administered to our guinea-pigs a glycerin enema, 

 after which we introduced into the rectum from 

 I to 20 c.c. of milk. About a month afterwards we 



