56 ANAPHYLAXIS AND ANTI-ANAPHYLAXIS 



authors, fearing the recurrence of these symptoms, 

 decided first to vaccinate their animals by our method. 

 In order to test the efficacy of the latter, they divided 

 the animals into two groups. The first group, com- 

 posed of ninety animals, was given subcutaneously 

 a preventive inoculation of i c.c. antiserum. The 

 second group, which served as controls, was given 

 no preliminary inoculation. Five hours after, all the 

 animals of the two groups were given an injection 

 of 5 c.c. of antiserum and 0-5 c.c. of bacillary emulsion. 



The results of this experiment are quoted verbatim 

 from the author's account: " No symptom of ana- 

 phylaxis, however sUght, was observed during the 

 twenty - four hours following sero - vaccination in 

 the ninety animals which had undergone the pre- 

 liminary anti-anaphylactic injection. On the con- 

 trary, ten animals (out of ninety) in the group used 

 as controls exhibited anaphylactic symptoms, which 

 consisted in oedema of the muzzle with hypersaliva- 

 tion, or in oedema of the mucous membranes of the 

 vulva and anus, accompanied by colic." 



The surprising rapidity with which the anti-anaphy- 

 lactic state is established is a point of the greatest 

 utility. It has enabled us to carry out, in a short 

 time, a whole series of vaccinations, to which we 

 attach the name of ** subintrant," which confer on the 

 animal an immunity that is proof against every 

 test. 



The small dose of serum which, in the experiment 

 quoted above, did duty as vaccine, only safeguards, 

 as we have remarked, against one or two lethal doses 

 of serum. 



But there are some cases where it is necessary to 

 provide protection against several lethal doses. In 

 a good number of serious infections we find ourselves 

 compelled to administer massive doses of serum to 

 subjects sensitised by former injections, and in order 



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