60 ANAPHYLAXIS AND ANTI-ANAPHYLAXIS 



we had animals vaccinated several times subcutane- 

 ously which afterwards withstood the most severe 

 inoculations made intraperitoneally, intracerebrally, 

 intrathecally, or intravenously. 



If desirable, we can make use of several routes 

 together for purposes of vaccination. We can begin, 

 for example, with a subcutaneous injection, then an 

 intravenous injection, and finally an intraspinal injec- 

 tion. An animal thus vaccinated, no matter to what 

 point of economy we go in use of the serum, after- 

 wards resists multiple lethal doses. 



Are the phenomena of local anaphylaxis liable tO' 

 occur if the same process be used ? The first re- 

 searches in this direction were carried out by our 

 collaborator Grineff.* This author sensitised rabbits 

 with heated egg-albumen, these animals being, as we 

 know, the most suitable for local anaphylaxis. 

 Starting from the fourth subcutaneous injection,, 

 Grineff observed characteristic cutaneous lesions. 

 These lesions were most marked at the time of the 

 final injections. In order to prevent these lesions, 

 the author injected the auricular vein of two rabbits, 

 the day before the fourth subcutaneous injection, 

 with 2 c.c. of solution of egg-albumen. The day 

 following this vaccination he made the fourth injec- 

 tion of ID c.c. egg-albumen, inoculating at the same 

 time two control rabbits with the same quantity of 

 egg-albumen . The two control rabbits exhibited a few 

 days after marked infiltration of the skin, while the 

 two other vaccinated rabbits exhibited nothing 

 abnormal. The same phenomenon was observed' 

 after the fifth or the sixth subcutaneous injection. 

 The two controls after eachinjectionhad a large amount 

 of oedema proceeding to necrosis, whilst the two other 

 rabbits, which had been given the day before an anti- 

 anaphylactic intravenous injection, remained unhurt, 

 1 Comptes rend. Soc. de Biol., Ixxii., p. 974, 1912. 



