62 ANAPHYLAXIS AND ANTI-ANAPHYLAXIS 



a few minutes before, a weak dose of the same blood 

 (o-2 to 0'5 CO.) to make sure of preserving the rabbit 

 from death. 



This experiment, we may say in passing, permits 

 of very powerful hsemolytic sera being obtained 

 (i : 6,000) with 3 to 4 injections of blood intra- 

 venously, without the animal running the least 

 danger. What is most important is that by this 

 means we can obtain, without any loss of animals^ 

 antibacterial and anti-endotoxic sera^ by injecting 

 bacterial cultures intravenously. Those who are in 

 the habit of immunising horses do not ignore the 

 serious risks which attend the intravenous method 

 of injection. It is by no means rare to see horses 

 fall to the ground a few minutes after the injection, 

 and to experience afterwards much difficulty in 

 recovering from the shock. Very often they do not 

 recover. Cases of sudden death of horses are no 

 longer taken into account. Under certain conditions 

 precisely similar symptoms are observed in rabbits. 



We tried at first to prevent anaphylactic mishaps 

 in rabbits' by applying in their case the method of 

 small doses. We very soon demonstrated the fact 

 that rabbits injected with meningococci intraven- 

 ously were with certainty protected from fatal con- 

 sequences by the use of this method. 



On the strength of this result, the constancy of 

 which we have had occasion to verify a number of 

 times, we commissioned our collaborator L. Cruveil- 

 hier^ to apply this process to some female goats in 

 immunising against the gonococcus and the diphtheria 

 bacillus.^ Experience shewed that female goats, when 

 immunised intravenously, after a preliminary injec- 

 tion, have never exhibited grave symptoms. The 



1 Annates de I'Institut Pasteur, xxvi., p. 83, 1912. 



2 Comptes rend. Soc. de Biol., Ixvii., p. 266, 1909. 



3 Ibid., Ixix., p. 38, 1910. 



