VACCINATING INJECTION 71 



cases for serum. When we wait forty-eight hours, 

 reckoning from the injection of serum, we find an 

 anti-anaphylactic state which did not exist the 

 evening before. 



We can therefore assert, on the basis of our experi- 

 ments on guinea-pigs, that, whatever may be the 

 sensitising substance, whether it be milk, egg-albumen, 

 or even serum, the anaphylactic state can be abolished 

 by the administration of this substance, either by 

 the rectum or by the m^outh. 



After these experiments we tried to effect anti- 

 anaphylaxis by the mouth with other substances as 

 well. Our collaborator Grineff has succeeded in 

 obtaining it with heated egg-albumen;^ Ch. Richet^ 

 has obtained it with crepitin. 



We have seen above that egg-albumen, when 

 heated, acts from the point of view of anaphylaxis 

 in quite a different way from raw egg-albumen. It 

 became, therefore, interesting to see how heated egg- 

 albumen acted from the point of view of anti-ana- 

 phylaxis. Acting on our advice, Grineff proceeded to 

 vaccinate by the oral method with heated egg- 

 albumen, and he has arrived at the same results as 

 those obtained by us with milk, serum, and raw 

 egg-albumen. 



Ch. Richet, to whom we are indebted for the infor- 

 mation on the subject of alimentary anaphylaxis, 

 likewise stated that the animal could be rendered 

 anti-anaphylactic per os. He related the case of a 

 dog which received crepitin by the mouth, and 

 which two days after withstood a toxic dose injected 

 intravenously without symptoms. 



To sum up, we can produce an anti-anaphylactic 



condition — that is to say, we can prevent anaphylactic 



shock from occurring — ^by the following different 



* Comptes rend. Soc. de Biol., Ixxii., p. 344, 1912. 

 ^ Ibid., Ixx., p. 252, 191 1. 



