• ADDITIONAL EXAMPLES OF ANAPHYLAXIS 7& 



Guinea-pigs are injected with tissue extracts of the 

 horse, of the sheep, or of man. After the prescribed 

 interval, they are tested intravenously. The test is 

 carried out with the organic extract that has been 

 used at the first injection or with extracts of other 

 organs, or, better still, with serum from the cor- 

 responding animal. 



The result of these experiments, which were first 

 carried out by Ranzi,^ shews that guinea-pigs can be 

 sensitised by tissue extracts, but that the anaphylactic 

 state thus produced is not specific. For instance, the 

 animals sensitised with a given organ react, at the 

 time of the intravenous test, not only to the extract 

 of this organ, but also to that of another organ. 

 They also react equally well to the serum of the same 

 animal. 



Ranzi observed, moreover, that animals sensitised 

 to sheep's serum are equally hypersensitive to the 

 tissue extracts of the sheep . 



In view of these facts our late lamented co-worker 

 Ohkubo^ debated whether in the experiments indi- 

 cated it was not simply a question of anaphylaxis in 

 the presence of serum contained in the organs rather 

 than of anaphylaxis in the presence of the organs 

 themselves. This idea appeared to him as all the 

 more probable from the fact that guinea-pigs, when 

 sensitised with organic extracts of the rabbit, reacted 

 in consequence more vigorously to rabbit's serum 

 than to the organic extracts in question. 



In order to verify this hypothesis, Ohkubo made 



We bear in mind the fact that the procedure of anti-anaphylactic 

 vaccination in small doses, applied to the red corpuscles, permits 

 of the avoidance of accidents which are often such a troublesome 

 obstacle to the preparation of hsemolytic sera when the inj ection 

 is made intravenously. For details as to this point, see Coniptes 

 rend. Soc. de Biol., Ixvii., p. 266, 1909. 



1 Zeitschr. f. Immuniidtsf., I. Orig., ii., p. 12, 1909. 



2 Ibid., vi., p. 176, 1910. 



