18 



t'MARLES F. W. MC CLURE 



A marked difference is observed also between the pH value 

 of the lymph obtained from the ligated legs and that found 

 in the coelom. The greater acidity of the lymph from the 

 Ligated area (pH, 4.97) is apparently associated with meta- 

 bolic changes in the muscles, which for a considerable period 

 of time have been completely shut off from the general cir- 

 culation. The pH value of the coelom lymph was found to 

 be essentially normal (pH, 7.60) ; the reason for this is, appar- 



TABLE 4 



Behavior of oedematous gastrocnemius muscle from ligated leg of frog, in 

 Ringer's solution approximately isotonic, with normal resting muscle. 



Control: Behavior of gastrocnemius muscle from unligated leg of same frog, 

 in Singer's solution- approximately isotonic with normal resting muscle. 



Ringer's solution: A = 0.445° — pH, 7.0. Room temperature. 



ently, that in all parts of the body not shut off by a ligature 

 from the general circulation, waste products are eliminated 

 in the usual way. The fact that lymph taken from one part 

 of an oedematous frog gives an essentially normal reaction, 

 while lymph taken from another part of the same frog gives 

 an acid reaction, is evidence of the fact that acidity does not 

 in all forms of oedema invariably play the essential role, as 

 is maintained by Martin Fischer. From the investigations of 

 Loeb ('98) we know that muscles may swell and increase in 



