34 



CHARLES I'. W. 1IC CLUltK 



esting to observe the effects produced on muscles obtained 

 from oedematous red-leg frogs, when immersed in an acidu- 

 lated Ringer's solution approximately isotonic with normal 

 resting muscle. 



TABLE 9 



Behavior of gastrocnemius muscles from frogs with red-leg disease, in lymph 

 obtained from some frogs. 



Controls: Behavior of gastrocnemius muscles from same frogs, in Ringer's solu- 

 tion approximately isotonic with normal resting muscle. 



Finger's solution: A = 0.445° — pH, 7.0. Boom temperature. 



EXPERIMENT 



661 



LEFT GASTROCNEMIUS IN LYMPH 



(LEG sinus) 



Oram (percent) 



0.50 (0) 



0.51 (+2.0) 



0.51 (+2.0) 



0.52 (+4.H) 



0.52 (+4.0) 



0.50 (0) 



control: right gastrocnemius 

 in ringer 



Oram {per cent) 



0.49 

 0.48 

 0.47 

 0.43 

 0.41 

 0.41 



(0) 



(-2.0) 



(-4.0) 



(-12.2) 



(—16.3) 



(-16.3) 



685 



Lymph (coelom) 



0.31 

 0.30 

 0.29 



0.28 

 0.28 



(0) 

 (-3.2) 



(-6.4) 

 (-9.6) 

 (-9.6) 



Four experiments of this character are shown in table 10. 

 In two of the experiments, the pH value of the Ringer's solu- 

 lion was 6.5 and in the remaining two it was 4.8. In each 

 experiment the muscles were taken from the same frog. The 

 writer found that the behavior of the muscles in acidulated 

 Ringer was the same as that of the controls which were im- 



