126 THE PROTEINS AND PROTEASES [ch. 



the free state : combined with a small amount of acid as a salt, it is 

 insoluble in water but soluble in neutral salt solution, that is, it has the 

 solubilities of a globulin (see p. 134). 



Expt. 124. The formation of salts hi/ edestin. Grind up 5 gms. of seeds of the 

 Hemp {Cannabis sativa) in a coffee-mill. Extract with 50 c.c. of warm (not above 

 60° C.) 10% sodium chloride solution and filter. Add a drop of strong hydrochloric 

 acid to the filtrate. Edestin chloride, which is insoluble in salt solutions, is precipi- 

 tated. Filter and drain off" all the liquid, and then suspend the precipitate in 

 distilled water. Add 1 or 2 drops of hydrochloric acid carefully and stir till most or 

 all of the precipitate goes into solution. Filter, and to the filtrate add a few drops 

 of saturated sodium chloride solution. The edestin acid salt is again precipitated. 



The following is a list of the principal known globulins (Osborne, 2): 



/ Pea (Pisum sativum). 



T- . . 1 /. Broad Bean ( Vicia Faba). 



Legumin, in seeds of Itt , i /rr- • • x 



° Vetch ( Viaa sativa). 



\ Lentil {Ervum Lens). 



Vignin, in seeds of Cow Pea ( Viqna sinensis). 



Glycinin, in seeds of Soy Bean {Glycine hispida). 



r Kidney Bean {Phaseolus vulgaris). 

 Phaseolin (crystalline), in seeds of \ Adzuki Bean (P. radiatus). 



[Lima Bean {P. lunatus). 

 Conglutin, in seeds of Lupin (Lupinus). 



rPea (Pisum sativum). 

 Vicilin, in seeds of } Broad Bean ( Vicia Faba). 



[Lentil {Ervmn Lens). 

 Corylin, in seeds of Hazel Nut ( Cori/ius Avellana). 



(Almond (Prunus Amygdalus). 



Amandin, in seeds of . 



1 Peach (P. Persica). 

 Plum (P. domestica). 

 Apricot (P. Armeniaca). 

 [European Walnut {Juglans regia). 



Juglansin, in seeds of \ American Black Walnut (J. nigra). 



[American Butter-nut (/. cinerea). 

 Excelsin (crystalline), in seeds of Brazil Nut (Bertholletia excelsa). 



Edestin, in seeds of Hemp (Cannabis sativa). 



Avenalin, in seeds of Oat (Avena sativa). 



Castaiiin, in seeds of Sweet Chestnut (Castanea vul- 

 garis). 



Maysin, in seeds of Maize (Zea Mays). 



Tuberin, in tubers of Potato (Solanum tuberosum). 



