26 MICRO-DUG ANISMS IN WATER 



only to a temperature of from 58-65 C. for 1-2 hours 

 on five to eight successive days. At this temperature 

 no coagulation of the albumen takes place, as is the 

 case at higher temperatures, and the milk is at the same 

 time perfectly sterile, and can be preserved for an in- 

 definite length of time. See also p. 5 for the prepara- 

 tion of milk-serum by filtration. 



The composition of some of the culture liquids 

 which have been extensively employed in special in- 

 vestigations are now appended : 



Pasteur s Solution. 1 To 100 parts of water add 1Q 

 parts candy sugar, 1 part ammonium tartrate, and the 

 ash of 1 part yeast. Bucholtz substituted for the yeast 

 ash 0*5 grm. of potassium phosphate. 



Colin s Solution? To 100 c.c. of distilled water add 

 I'O grm. ammonium tartrate, *05 grm. of tricalcium 

 phosphate, '5 grrn. potassium phosphate, '5 grm. crystal- 

 lised magnesium sulphate. 



Naegelts Solutions? (1) To 100 c.c. of water add 

 1 grm. ammonium tartrate, O'l grm. of dipotassium 

 phosphate (K 2 HP0 4 ), 0'02 grm. of magnesium sulphate 

 (MgS0 4 ), O'Ol grm. of calcium chloride (CaCl 2 ). Instead 

 of the ammonium tartrate, ammonium acetate, am- 

 monium lactate, asparagin, or leucin may be added. 



(2) To 100 c.c. of water add 1 grin, of egg-albumen 

 peptone or soluble albumen, 0'2 grm. of dipotassium 

 phosphate, 0'04 grm. of magnesium sulphate, 0'02 grm. 

 of calcium chloride. 



(3) To 100 c.c. of water add 3 grms. of cane sugar, 

 1 grm. of ammonium tartrate, and mineral substances- 

 as in No. 2. 



Percy Franklands Solutions. In the study of the 



1 Annales de Cliim. et Pliys. Iviii. 323. 



2 Beitrdgc zur Biologic d. Pfl.anzen, i. 195. 



3 Untersuchungen uber niedere Pilzc, 1882, i. 



