DETECTION OF PATHOGENIC BACTEEIA IN WATER 283 



300-350 grms. in weight, but for a larger animal a 

 larger dose must be employed. The injection is followed 

 by a rapid fall in temperature, ultimately resulting in 

 death. According to Koch, the cholera bacilli are the 

 only spirilla which have yet been discovered in the 

 course of cholera investigations which give anything 

 even approaching to these symptoms when the above 

 quantity is employed. 



Sanarelli (loc. cit.\ however, recently isolated no- 

 less than thirty-two vibrios from water, morphologically 

 distinct from each other, all of which gave the indol- 

 reaction and some the cholera-red-reaction, four of 

 which not only exhibited both these reactions, but were 

 extremely pathogenic to animals, producing symp- 

 toms undistinguishable from those considered typical 

 of cholera infection. Sanarelli- is therefore of opinion 

 that .many varieties of vibrios may exist in water,, 

 morphologically distinct from the cholera vibrio, but 

 capable of producing a disease in man and animals in 

 its morbid aspects identical with cholera, and that the 

 assumption that this disease is produced by only one 

 particular variety of vibrio, as hitherto held, must be 

 abandoned. 1 



DETECTION OF ANTHEAX SPORES IN WATER 



A method has been devised by one of us 2 suitable for 

 the detection of anthrax spores when present along with 

 other micro-organisms in water. A large proportion of 

 the organisms present in water, and more especially those 

 causing liquefaction of the gelatine, are very sensitive 

 to a temperature even considerably below that of boil- 



1 In this connection see also a more recent paper by Dunbar, ' Versuche 

 zum Nachweis von Cholera vibrionen in Fluszwasser,' Arbeiten a.d. 

 Kaiserl. Gesundheitsamte, vol. ix. 1894, p. 379. 



2 ' Experiments on the Vitality and Virulence of Sporiferous Anthrax 

 in Potable Waters,' Percy Frankland, Proc. Boy. Soc., 1893, p. 192. 



