o66 MICRO-ORGANISMS IN WATER 



absolutely parallel and comparable experiments with or- 

 dinary anthrax bacilli and asporogene bacilli respectively 

 in both blood and bouillon, and that he confines him- 

 self to the bare statement that both these varieties of 

 anthrax bacilli exhibit the same degree of resistance. 



Desiccation Experiments in Sunlight with Broth Cultures 

 of Asporogene Bacilli 



The dried bouillon cultures were prepared as pre- 

 viously described. The maximum duration of life of 

 the bacilli in contact with air = 5 to 5J hours. In 

 vacuo under similar conditions, maximum duration 

 = 6i hours. The culture employed was only twenty- 

 four hours old, and it was found that the younger the 

 culture the greater was its resistance. The temperature 

 recorded during the period of insolation varied between 

 25 and 35 C. 



Desiccation Experiments in Sunlight with Anthrax 

 Blood 



(1) Maximum duration of the bacilli in contact 

 with air 8 hours. The development of the last culture 

 was delayed for forty-eight hours. 



In vacuo under similar conditions, maximum dura- 

 tion=ll hours. The development of the last culture 

 was delayed for twenty-four hours.. In both cases the 

 bouillon cultures obtained from the insolated blood 

 killed guinea-pigs in thirty-six hours. In another series 

 of experiments bits of sterilised blotting-paper were 

 soaked in anthrax blood and insolated in test-tubes. 

 This blotting-paper, insolated for from one, two, three, 

 up to fifteen hours, introduced under the skin of guinea- 

 pigs killed them in from thirty-six hours to three days. 

 When insolated for sixteen hours this infected paper no 

 longer killed guinea-pigs, although in bouillon it gave 



