SPIRILLA 405 



VIBKIO AUREUS 



Authority. Weibel, ' Untersuchungen iiber Vibrionen,' Centralblatt /. 

 Bakteriologie, vol. iv., 1888, pp. 225, 257 and 289. 



Where Found. In sewer mud. 



Microscopic Appearance. About ItL time as thick as the Comma spirillum. 

 Typical comma and ' S ' forms visible, with rather plump and blunted ends ; 

 also gives rise to longer and shorter forms, the latter being sometimes even 

 oval. The tendency to become spiral appears to be less pronounced than in 

 most spirilla Degenerative forms are also found. It is not motile. It is not 

 coloured by Gram's method. 



Cultures. 



GELATINE PLATES. Grows in the depth, and on the surface forms flat 

 expansions of a pure yellow-gold colour. Under a low power the surface 

 colonies are circular, smooth-rimmed, granular, golden yellow in the centre, 

 and paler towards the edge. The depth colonies are ovoid and rather 

 coarsely granular ; the centre is golden yellow to start with, and the periphery 

 lighter. Later on a spindle-shaped thickened portion appears in the centre, 

 which later becomes brown and quite black, whilst on both sides the golden 

 yellow edge is visible. No liquefaction ensues. 



GELATINE TUBES. Forms a rather thick finely granular growth along the 

 needle's path in the depth. It forms a round bowl-shaped expansion on the 

 surface. Both in the depth and on the surface the colour is yellow ochre. 



AGAR-AGAB. Grows only on the surface, forming a dirty white expansion in 

 which raised spots of a yellow colour become visible ; finally the growth 

 becomes 2 mm. thick, and forms a uniform pasty covering of a golden yellow 

 colour. 



POTATOES. Grows abundantly, forming a thick pasty golden yellow ex- 

 pansion. 



BKOTH. Benders the liquid turbid and forms a deposit, but no pellicle. 



VIBRIO FLAVUS 



Authority. Weibel, ' Untersuchungen iiber Vibrionen,' Centralblatt f. 

 Bakteriologie, vol. iv., 1888, p. 260. 



Where Found In sewer mud. 



Microscopic Appearance. Besembles Vibrio aureus. 



Cultures. 



GELATINE PLATES. The shape of the colonies resembles Vibrio aureus ; the 

 colour on a dark ground is dirty greyish yellow, on a white , ground straw 

 yellow. Under a low power the depth colonies are golden yellow, but with no 

 dark centre, and are very finely granular. The surface colonies are pale 

 yellow, with dull grey washed-out spots ; the periphery has usually a white 

 zone. No liquefaction ensues. 



GELATINE TUBES. Besembles Vibrio aureus. 



AGAR-AGAR. Besembles Vibrio aureus, only produces a yellow ochre 

 colour. 



POTATOES. Besembles Vibrio aureus, only produces a yellow ochre colour. 



BROTH. Besembles Vibrio aureus. 



