BACILLI KESEMBLING TYPHOID BACILLUS 413 

 BACILLUS AQUATILIS SULCATUS I. 



Authority. Weichselbaum, Das osterreichische Sanitatswesen, 1889, 

 Nos. 14 to 23. 



Where Found. In the Vienna ' Hochquellenleitungswasser ' at the time 

 of the introduction of the water obtained from the Schwarza, a little river in the 

 vicinity of the Scemmering. 



Microscopic Appearance. Small rods resembling the typhoid bacilli. Is very 

 motile. No spores observed. Is not stained by Gram's method. 



Cultures. 



GELATINE PLATES. Circular superficial colonies appear on the plates after 

 two days, the periphery of which is very thin and bluish in colour, whilst the 

 centre is thicker and whitish. The edge is distinctly serrated. Under a low 

 power the surface is seen to consist of numerous fine lines crossing one another 

 from various parts, resembling the typhoid colonies. The edge of the colony 

 is white and the centre yellowish. Later on the lines in the centre become 

 much more marked and intricate, whilst the periphery remains white and of 

 the same appearance as before, but after four days the whole colony becomes 

 yellow. No liquefaction of the gelatine takes place. 



GELATINE TUBES. Exhibits a flat white expansion on the surface, with ser- 

 rated edge, after twenty-four hours ; it looks thicker than in the typhoid growth, 

 and is more restricted than the latter. 



AGAR-AGAR. Produces at 37*5 C. a somewhat thick white expansion, 

 having an odour of whey. 



POTATOES. No growth is visible at 37'5 C., the point of inoculation only has 

 a moist appearance. At the temperature of a room this is also the case at first, 

 but later a very thin, shining and moist expansion of a cream colour becomes 

 visible. The potato in the vicinity of the growth sometimes becomes of a 

 blue grey colour. 



Remarks. It grows far more rapidly than the typhoid bacillus at the temperature 

 of the room, but only develops scantily at 37 C. It produces a visible growth at. 

 from 5 to 7 C., at which temperature the typhoid bacillus cannot develop. 



BACILLUS AQUATILIS SULCATUS II, 



Authority. Weichselbaum, Das osterreichische Sanitatswesen, 1889,. 

 Nos. 14 to 23. 



"Where Found In the Vienna ' Hochquellenleitungswasser ' at the time 

 when the water from the river Schwarza was introduced. Also by Tataroff in 

 Dorpat well water (Die Dorpater Wasserbacterien, 1891, p. 31). 



Microscopic Appearance. Short rods with rounded ends resembling the 

 smaller typhoid bacilli. Is motile. No spore-formation observed. It is not 

 coloured by Gram's method. 



Cultures. 



GELATINE PLATES. The surface colonies resemble at the end of two days 

 those of the typhoid bacillus and the B. aquatilis sulcatus I., but they are some- 

 what thicker and the edge is not visibly serrated. With a low power the periphery 

 is seen to be slightly serrated, but the system of lines is not so clearly defined as 

 in the B. aquat. sul. I. The centre is yellow and the periphery white. At the 

 end of three days the colonies have become thicker and no signs of lines or 

 serration are visible under the microscope, and the colonies appear, with ex- 

 ception of the outer zone, quite yellow in colour. 



GELATINE TUBES. Rather thicker and more restricted ; otherwise like B. 

 aquat. side. I. 



BROTH. The liquid becomes turbid in from twenty-eight to thirty- seven 

 hours ; a deposit is formed at the end of two days. 



AGAR-AGAR. At 37 C. a grey white expansion is visible in twenty-four hours. 



POTATOES. At room-temperature the point of inoculation at first exhibits 

 a yellow blue colour, but later a yellow grey or yellow brown expansion makes 

 its appearance, which is at times very luxuriant. A faint smell of urine attends 

 its growth. 



Remarks. In respect of temperature it resembles B. aquatilis sulcatus I. 



