414 MICRO-ORGANISMS IN WATER 



BACILLUS AQUATILIS SULCATUS III. 



Authority. Welch selbaum, Das osterreichische Sanitatswesen, 1889, 

 Nos. 14 to 23. 



Where Found. In the Vienna ' Hochquellenleitungswasser ' at the time 

 when the water from the river Schwarza was introduced. 



Microscopic Appearance. Very short rods, often resembling cocci. Is 

 very motile. No spores observed. Is not coloured by Gram's method. 



Cultures. 



GELATINE PLATES. The surface colonies are visible in from two to three 

 days as fine discs, thicker in the centre, which is white, than at the periphery 

 which is serrated, very thin and bluish in colour. Under a low power the 

 system of lines is very clearly defined. As they become older, the colonies 

 become larger and thicker, lose their bluish tint, and the lines are replaced by 

 a much closer medley of short lines and furrows, whilst the colonies assume 

 a yellowish colour. There is nothing characteristic about the depth colonies. 



GELATINE TUBES. Forms in twenty-four hours a very thin white expansion 

 with serrated edge, which rapidly reaches the walls of the tube. It emits a 

 very disagreeable smell. The gelatine is not liquefied. 



AGAR-AGAR. Forms at 37 C. an abundant grey white expansion,which smells 

 like serum. 



BROTH. The cultures have a most unpleasant odour. 



POTATOES. At room-temperature it produces an abundant growth, bright 

 yellow in colour. The edge of the expansion is irregular in contour. At the end 

 of nine days the whole potato in the vicinity of the growth assumes a bluish 

 green colour. 



Remarks. In respect of temperature it resembles B. aquatilis sulcatus I. 



BACILLUS AQUATILIS SULCATUS IV. 



Authority. Weichselbaum, Das osterreichische Sanitatswesen, 1889, Nos. 

 14 to 23. 



Where Found. In the Vienna ' Hochquellenleitungswasser ' at the time 

 when the water from the river Schwarza was introduced. 



Microscopic Appearance. The length of the individual bacilli varies 

 according to the medium in which they are cultivated. In broth they give rise 

 to threads. The shorter bacilli are motile, but not the threads. No spore forma- 

 tion observed. They are not stained by Gram's method. 



Cultures. 



GELATINE PLATES. The surface colonies are visible on the fourth day, and 

 are at first very thin and of a bluish colour with serrated edge and a white and 

 rather thicker centre. Under a low power the characteristic arrangement of 

 lines previously mentioned is visible, the smaller colonies are white and the 

 larger ones yellow in the centre. Later the whole colony becomes of a yellow 

 colour and looks larger and thicker, whilst in the place of the lines irregular 

 furrows make their appearance. The depth colonies are round and yellow. 

 The gelatine is not liquefied. 



GELATINE TUBES. Grows slowly ; later a somewhat thin grey white expan- 

 sion, with serrated edge, gradually extends over the gelatine surface up to the 

 walls of the tube. 



AGAR-AGAR. At room-temperature gives rise after two days to a grey white 

 expansion; at 37 C., however, it grows with difficulty, even after six days there 

 being only a scanty development. 



POTATOES. No growth. 



