BACILLI 



BACILLUS RUBEFACIENS 



Authority. Zimmermann, Die Bakterien unserer Trink- und Nutzwasser, 

 insbesondere des Wassers der Chemnitzer Wasserleitung, Chemnitz, 1890. 



Where Found. In the Chemnitz water supply. 



Microscopic Appearance. Slender bacillus about 0-32 n broad and 0-75 

 to 1-65 fj. long, with rounded ends. Occurs in pairs or more, and is often found 

 lying side by side. It is very motile. 



Cultures. 



GELATINE PLATES. In the depth round or bean-shaped colonies are visible, 

 whitish in colour, with a touch of yellow red. The surface colonies form flat 

 expansions, greyish in colour, with a suggestion of red. Under a low power the 

 depth colonies are circular, granular, smooth-rimmed, yellowish or brownish in 

 colour. No liquefaciion ensues. 



GELATINE TUBES. Forms a somewhat thick w r hitish grey expansion, which 

 later becomes yellowish. The gelatine between the growth and the walls of the 

 tube assumes a bluish white opalescence. Develops well in the depth. In old 

 cultures the gelatine often becomes of a light wine-red colour. When streaked 

 it forms a thin bluish grey shining expansion, with a delicately serrated edge ; 

 later it becomes somewhat folded. 



AGAR-AGAR. Forms a smooth bluish grey raised expansion. 



POTATOES. Forms a fairly abundant greyish yellow, later brownish red, 

 expansion, which on the second day renders the potato pinkish. 



DER EOTHE BACILLUS' 



LIQUEFIES GELATINE | 



Authority. Lustig, Diagnostic der Bakterien des Wasscrs, 1893, p. 72. 



"Where Found. Found on one occasion in river-water. 



Microscopic Appearance. Small bacillus with rounded ends, generally two 

 or three times as long as broad, but its dimensions and arrangement vary- 

 according to the temperature and material used for its cultivation. It is very 

 motile ; the filaments are also motile When the cells become more coloured its 

 movements become oscillatory only. Forms spores. 



Cultures. 



GELATINE PLATES. In forty-eight hours the surface colonies resemble grey 

 dots with a red centre. Under a low power they are circular with serrated 

 edge and granular surface, whilst the centre is a raspberry-red colour. The 

 gelatine becomes liquefied, the colony sinks, and the red colour extends in all 

 directions. In from four to six days the whole plate is fluid. 



GELATINE TUBES. At the end of twenty-four hours a small funnel-shaped 

 depression is visible at the point of inoculation, in the centre of which the 

 characteristic pigment is found. A thin transparent thread of dirty white liquid 

 marks the needle's path in the depth. The liquefaction proceeds until finally 

 the whole contents of the tube are fluid, consisting of a slimy glutinous 

 material, raspberry-red in colour. 



AGAR-AGAR. At the ordinary temperature a moist, crimson lake, and shining 

 expansion appears, but at 37 to 40 C. only a milk-white growth is visible, which, 

 even when kept for weeks, does not become red. 



POTATOES. Grows rapidly, producing a sticky, slimy, raspberry-red expansion 

 which covers the whole surface, and later assumes a metallic colour. 



BROTH. Benders it turbid and produces a red pigment at the ordinary 

 temperature. 



STERILISED DISTILLED WATER. It does not develop, and the water remains 

 quite clear. On examining this water in suspended drops motionless shining 

 bacillar forms with refracting protoplasm are visible. After thirty days this 

 water, when inoculated into gelatine, gives rise to typical cultures of the bacillus. 



