BACILLI 467 



BACILLUS GEN. NOV. 





LIQUEFIES GELATINE 



Authority. - Trambusti and Galeotti, ' Neuer Beitrag zum Studiumder inne- 

 Ten Struktur der Bakterien,' Centralblattfiir Bakteriologie, vol. xi., 1892, p. 718 



Where Found. In drinking-water. 



Microscopic Appearance. When young the bacillus is about 3 to 5 /* long, 

 .and with somewhat flattened ends ; later it reaches 8 to 9 /u in length, whilst 

 still later elliptical rings appear in the interior of the rod, which are at first 

 attached end to end, but which later become separated and appear as if they 

 were free within the filament ; finally the filament bursts and the elliptical 

 forms escape. These free oval forms stain more intensely at the periphery, and 

 are about 1*5 fj. long and 0'9 n broad. From these oval forms the bacillar stage 

 recommences. In broth cultures kept at 37 C. for three or four days, these 

 various stages are best watched. These oval forms behave differently from 

 spores, and are not regarded as such by the authors. An aqueous alcoholic 

 solution of safranin was employed for staining purposes. Both the bacillar 

 .and oval forms exhibit slow rotatory movement. 



Cultures. 



GELATINE PLATES At 20 C. in forty-eight hours there appear circular grey 

 granular colonies with slightly irregular contour, and surrounded by a liquid 

 :zone. 



GELATINE TUBES. The gelatine is rapidly liquefied, so that in twenty-four 

 hours the whole contents of the tube are fluid. 



AGAR-AGAR PLATES. At 37 in forty-eight hours slightly raised circular 

 colonies with serrated edge are produced, from which sometimes serpentiform 

 processes appear which become longer the farther they are from the centre. 



AGAR-AGAR TUBES. Grows abundantly on the surface, but scantily in the form 

 of aggregations of dot-shaped colonies along the needle's path in the depth. 

 On sloped agar surfaces a luxuriant growth appears after twenty-four hours, 

 grey white in colour, rugose and brittle, which completely covers the surface. 



POTATOES, Forms along the needle streak a dirty grey, dry and raised 

 growth. 



BROTH. At 37 after twenty-four hours a grey white rugose skin forms on 

 ihe surface, whilst the remainder of the liquid remains quite clear, and retains 

 its alkalinity. 



MILK. Milk is rapidly coagulated, and the liquid portion exhibits a strongly 

 acid reaction. 



BACTERIUM GKAVEOLENS 



LIQUEFIES GELATINE 



Authority. Bordoni-Uffreduzzi, Fortschritte der Medicin, 1886, p. 157. 



"Where Found. On the epidermis between the toes. Found by Tils 

 several times in the Freiburg water supply. 



Microscopic Appearance. Small bacilli about 0-8 /t long and nearly as 

 broad. No spore formation observed. 



Cultures. 



GELATINE PLATES. Forms irregular greyish white spots which rapidly liquefy 

 the gelatine, giving rise to an offensive odour resembling that from feet. Later 

 it becomes of a greenish yellow colour. 



POTATOES. Forms grey centres with a most offensive odour. 



BLOOD SERUM. Liquefies the serum. 



H H 2 



