BACILLI 469 



BACILLUS 'AMYLOZYME' 



Authority. Perdrix, ' Sur les Fermentations produites par un Microbe 

 anaerobic de 1'Eau,' Annales de Vlnstitut Pasteur, vol. v., 1891, p. 287. 



Where Found. Very frequently in the water supply of Paris derived from 

 ihe Seine and from the Vanne. 



Microscopic Appearance. Bacillus about 2 to 3 /u. long and 0-5 n broad, with 

 rounded ends. Occurs in pairs and chains. It is motile, but the movements 

 become slower with the increasing length of the chain. It is anaerobic, and in 

 the presence of air its motility is diminished and even entirely checked. It 

 forms spores, which will withstand ten minutes at 80 C C. 



Cultures. 



ANAEROBIC GELATINE TUBES. At the end of from five to six days it forms small 

 white centres, round each of which are seen bubbles of gas which break up the 

 .gelatine, but without causing any liquefaction. 



POTATOES. After some days whitish isolated spots are visible, which become 

 larger and more circular, forming small nipple-shaped protuberances, in the 

 vicinity of which the potato becomes, depressed. The potato becomes partially 

 liquefied. On opening the tubes containing the cultures there is a slight ex- 

 plosion, and in consequence of the diminished pressure the colonies part with 

 the gas which they enclosed, each exhibiting tiny craters from which bubbles 

 of gas escape. 



BROTH. It grows in this medium for a short time. 



Remarks. It grows best at about 35 C. ; it will produce gas at 16 to 17 C. and 

 up to 43 C., but it will not grow at from 44 to 50 C. It ferments glucose, saccharose, 

 lactose, starch. 



BACILLUS GLAUCUS 



LIQUEFIES GELATINEj 



Authority. Maschek, Die Bakterien der Nutz- und Trinkwdsser, Adametz. 

 Vienna, 1888. 



"Where Found. In water. 



Microscopic Appearance. Fine slender bacillus of variable length. It is 

 not motile. No spore formation observed. 



Cultures. 



GELATINE PLATES. Forms circular, grey colonies. Under a low power they 

 are smooth-rimmed, and the centre is intensely grey, whilst towards the peri- 

 phery they are brown and have a radial folded appearance. On the eighth day 

 liquefaction begins, and the colony sinks. 



GELATINE TUBES. Grows quickly on the surface and in the depth, rapidly 

 liquefying the gelatine and forming masses of grey material. 



AGAR-AGAR. Grows quickly, producing a grey expansion. 



POTATOES. Forms a dirty white, later dark grey, expansion. 



