MICROCOCCI 489 



' SEIDENGLANZENDER BACILLUS ' 



Authority. Tataroff, Die Dorpatcr Wasserbaclerien, Dorpat, 1891, p. 26. 



Where Found. -In Dorpat well-water. Resembles very closely the B. can- 

 dicans obtained from soil and described by Percy and G. C. Frankland (loc. cit.). 



Microscopic Appearance. Ovoid-shaped bacilli about 0-8 n broad and 1-7 /i 

 long, with rounded ends. Grows also in pairs and forms short threads. Slight 

 oscillatory movements. 



Cultures. 



GELATINE PLATES. The depth colonies are small round white lustrous balls. 

 The surface colonies are button-shaped, milk-white, with a jagged edge, and 

 lustrous. Under a low power the depth colonies are circular, brownish yellow, 

 smooth-rimmed and granular. The surface colonies are brownish grey, granular, 

 opaque in centre, but lighter towards the edge, which is lobular and radially 

 striped. No liquefaction takes place. 



GELATINE TUBES. Forms a sword-like growth in the depth, and on the sur- 

 face a small white shining radially striped jagged basin-shaped expansion. 

 Later it becomes smooth and concave. 



AGAR-AGAR. Forms a rather thick white skin-like expansion, which has a 

 violet iridescent mother-of-pearl appearance. Later it becomes thinner and 

 fatty, although the iridescence is retained. In the condensed water a rather con- 

 siderable sediment collects. In glycerine-agar the skin is wrinkled, and when 

 it becomes flatter assumes a silk-like shining appearance. 



BLOOD SERUM. Forms here and there white wart-like growths. 



POTATOES. Forms a restricted dirty white delicate shining and smooth ex- 

 pansion, which later becomes light brown. In the incubator the surface 

 becomes wrinkled, but later smoother, with the characteristic silk-like shining 

 appearance. 



BROTH. Benders the liquid turbid and forms a considerable deposit. A 

 delicate iridescent skin forms on the surface, which sinks to the bottom, whilst 

 a new one forms on the surface. 



Mir.Horowns BISKRA 



| LIQUEFIES GELATINE_| 



Authority. Heydenreich, Pcndinskaia Jasna, Petersburg, 1888. 



Where Found. In water and air. Found in pus and serous exudations. 



Microscopic Appearance. Diplococcus, with a capsule ; as they sometimes 

 lie side by side in pairs, they recall the appearance of sarcina. In length they 

 are from 0-86 to 2 /* Not motile. 



Cultures. 



GELATINE TUBES. After forty-eight hours at from 20 to 21 C. a grey white 

 mass appears all along the needle's path in the depth, which also at times 

 consists of dot-shaped colonies. On the surface a circular, whitish yellow ex- 

 pansion forms. After from three to four days liquefaction ensues in the shape 

 of a funnel, which becomes wider, so that in fourteen days the whole of the 

 gelatine is fluid. 



AGAR-AGAR. At 37 C. a greyish or yellowish white expansion is visible in 

 twenty-four hours. The colour, however, is not constant, neither is the degree 

 of virulence of the cultivation. The growth has a shining appearance resem- 

 bling that of sealing-wax. 



POTATOES. At from 30 to 35 C. a white or yellow growth is visible on the 

 second day. On this medium involution forms are found frequently. 



Remarks. It grows best at about 30 C., and only very slowly at 15 J C. It will 

 not grow at 45 C., and cultures exposed to 60 C. for a quarter of an hour and to 

 100 C. for five minutes are destroyed. It is pathogenic to rabbits, dogs, fowls, 

 horses, and sheep. By rubbing in some of the cultures, swellings and ulcers have 

 been produced in man. 



