504 MICRO-ORGANISMS IN WATER 



MICEOCOCCUS UEEAE .(Pasteur) 



Authority. Pasteur, Comptes rcndus, vol. 1., 1860. Van Tieghem, Compt. 

 rend., vol. Iviii., 1864. Jacksch, Zeitsclirift f. physioloyische Chcmie, vol. v., 

 1881, p. 395. Leube and Grasser, Virchow's Archiv, vol. c., p. 556. 



Where Found. In decomposed ammoniacal urine, also in air. Found by 

 Tils (loc. cit.) in water. 



Microscopic Appearance. Cocci from 0-8 to 1-0 n in diameter ; occurs fre- 

 quently arranged as diplococci and in tetrads, also frequently in more or less 

 long chains. Grows also in the shape of zoogloea and rose- wreaths (Jacksch). 



Cultures. 



GELATINE PLATES. Forms small white shining spots having a mother-of- 

 pearl lustre, and a smooth surface and sharply rounded contour. At the 

 ordinary temperature of a room they reach in ten days' time the size of a six- 

 pence and are slightly raised above the level of the gelatine, and resemble drops 

 of wax. No liquefaction takes place. 



GELATINE TUBES. Forms a thin tough thread-like growth along the needle's 

 path in the depth. Old cultures have a smell of paste. 



It grows best in Jacksch's culture-fluid : in 1 litre ~ grm. magnesium sul- 

 phate, i grm. acid potassium phosphate, 5 grms. sodium potassium tartrate, and 

 5 grms. urea. 



Remarks. According to Jacksch, the most favourable temperature for its 

 growth is 30 to 33 C. ; below no growth takes place, but it can be kept for several 

 days at -13 C. without being destroyed. It converts urea into ammonium carbonate. 



MICEOCOCCUS VEESICOLOE 



Authority. Fliigge, Die Mikroorganismen, 1886, p. 177. 



"Where Found. Frequently in air. Found also frequently in water by Tils 

 (Zoc. cit.). 



Microscopic Appearance. Small cocci, arranged in pairs or in aggregates. 



Cultures. 



GELATINE PLATES. Forms in the depth white dot-shaped centres in twenty- 

 four hours, which in two days become yellow in colour. Under a low power 

 these are circular, smooth-rimmed, finely granular, and of an opaque yellowish 

 green colour. The surface colonies form large expansions, irregular in shape, 

 often four-cornered, with lobular projections. They are slimy, shining, and 

 yellowish green in colour, but in some lights they have a greenish or bluish 

 mother-of-pearl lustre. The centre of the colony is often raised. No lique- 

 faction takes place. 



GELATINE TUBES. Forms a mother-of-pearl expansion with an irregular 

 and frayed edge. In the depth small ball-shaped, yellow colonies make their 

 appearance. 



