228 THE MICROSCOPE. 



Some of the corpuscles will appear of a delicate yellow 

 colour, and threads are seen extending from the nucleus 

 to the envelopes. The preparation should be preserved 

 and mounted in glycerine. 



Picro-carmine and Aniline Dye. For glandular tissue, 

 none of the aniline dyes answer so well as iodine green, 

 used in the form of a 1 per cent, watery solution. 

 Stain the tissue in picro-carmine, wash it in distilled 

 water, acidulated with acetic acid, and stain it in a 

 solution of iodine green. As it acts rapidly, care must 

 be taken not to overstain. Wash the section in w r ater, 

 and then transfer it to alcohol ; finally clear with oil 

 of cloves. The washing should be done rapidly, as 

 the spirit dissolves out the green dye. All prepara- 

 tions stained with iodine green must be mounted in 

 dammar. 



Picro-carmine and Iodine Green. Stain a section of 

 the cancellated head of a very young bone (foetal bone) 

 in picro-carmine, wash it in distilled water, and stain 

 it with iodine green and mount in dammar. All newly- 

 formed bone is stained red; that in the centre of 

 the osseous trabeculse, the residue of the calcified car- 

 tilage in which the bone is deposited, is stained green. 

 Many of the bone corpuscles are also stained green. 



Ossifying cartilage, the back part of the tongue, 

 Peyer's Patch, solitary-glands, trachea, and bronchus, 

 may all be treated in the same way. In preparing the 

 skin, take a vertical section from the sole of the foot 

 of a foetus. The cuticle and superficial layers of the 

 epithelium are dyed yellow, the rete Malpighii green, 

 and the continuation of these cells can be traced into 

 the ducts of the sweat-glands, which are green, and form 

 a marked contrast to the red stained connective tissue 

 of the cutis vera ; through which they have to ascend 

 to reach the surface. The outer layer of the grey 

 matter of the cerebellum with Purkinge's cells is, 

 whon double stained, red, while the inner or granular 

 I'ayer is green. Logwood and iodine green stains the 

 mucous glands of the tongue (green), and the serous 

 glands, lilac logwood stain. 



Eosin and Iodine Green. Eosin is used as the ground 



