90 THE INOCULATION OF ANIMALS AND THEIR STUDY. 



and the several bacteria therein isolated, and pure cultures 

 made. 



After complete examination of the abdominal and pelvic 

 organs, by means of a thick pair of scissors the ribs are cut 

 off along the singed lines, the sternum turned up, and exam- 

 ination and cultures of the thoracic organs made in the same 

 way as for the abdominal organs. 



3. Alter the complete autopsy the animal should be inciner- 

 ated, and if this is not practicable, it should be kept bathed 

 at least for two hours in a 5 per cent, carbolic acid solution, 

 and finally boxed in quicklime before burial. 



Cultures from the human body at autopsies should be made 

 as described for lower animals. 



4. Cultures from secretions of living animals and man should 

 be made immediately upon their passage and must be always 

 collected in sterilized vessels. The examinations and the cult- 

 ures should be prepared forthwith, using agar, bouillon, and 

 serum as media. 



The Roux-Nocard Method of Culture and Observation. 



History. Recently observations made by Roux and Nocard 

 for the growth of microorganisms in culture in the live 

 animal have shown that a number of minute bacteria are 

 found associated with certain diseases, notably the pleuro- 

 pneumonia of cattle. These microorganisms require a much 

 higher power of the microscope than that generally in use to 

 bring them into view, a magnification of 2000 at least being 

 necessary. 



Technic. Small collodion flasks are made thoroughly 

 sterile, filled with blood from the suspected animal, and then 

 closed with sterile collodion. These tubes or flasks are after- 

 ward introduced into the abdominal cavity of live rabbits and 

 guinea-pigs, and allowed to remain for a few days, after which 

 they are taken out and examined, when the small motile 

 microorganisms as mentioned above will be discovered. 



Importance. This method of observation and cultivation 

 in the live animal body seems to open a large field for the 



