QUESTIONS. 123 



the Bacillus syphilidis it showed considerable difficulty in tak- 

 ing up the auilin colors, yet when stained according to Ehr- 

 lich's or ZiehPs method it very quickly parted with its colors 

 when washed in mineral acids, especially sulphuric acid, con- 

 trary to what happens in the case of the Bacillus tuberculosis. 

 When decolorized, however, by means of alcohol the Bacillus 

 syphilidis retained the dye for a considerable time. 



For the staining of sections the following method is recom- 

 mended : Place the section in a cold solution of anilin-water 

 gentian-violet for from twelve to twenty-four hours at the 

 room temperature, or for two hours at a temperature of 

 40 C. Wash a few minutes in absolute alcohol, then put 

 the section for some seconds into 1.5 per cent, solution of per- 

 manganate of potassium, pass rapidly (for one or two seconds) 

 into sulphuric acid solution, wash thoroughly in water, and 

 mount on xylol balsam. When stained by this method the 

 Bacillus syphilidis shows considerable resemblance to the 

 Bacillus tuberculosis, being of similar size and showing similar 

 refractive spots in the body of the cell. 



As mentioned above, this bacillus has not been successfully 

 cultivated artificially, and inoculations of animals have also 

 been barren of results. 



Streptococcus of Syphilis. 



Vanniessen, by collecting blood of syphilitic subjects, and 

 allowing same to coagulate in sterilized tubes, has been able 

 from the serum of this blood to cultivate a streptococcus which 

 he believes to be the etiological factor in syphilis. His 

 experiments, however, have failed of confirmation by others. 



QUESTIONS. 



When and by whom was the Bacillus leprx found ? Where is it found ? 



Describe the Bacillus leprse. Does it contain spores? How is it grown 

 artificially ? How does it stain ? 



How do you differentiate Bacillus leprse from the Bacillus tuberculosis by 

 staining? What animals are susceptible to the infection? 



Give a ready method for the diagnosis of a leprous ulcer or nodule, and 

 give a diagnosis of leprosy in a suspected case. 



Describe the Bacillus syphilidis of Lustgarten ? Where is it found? How 

 does it stain ? How decolorized ? How does it stain in tissue ? How does it 

 grow in artificial culture-media? 



Differentiate between Bacillus syphilidis and Bacillus tuberculosis. 



