16 



BO TA NIC A L MICRO TE CHNIQ_ UE. 



xylol may also be accomplished by means of the settling-cyl- 

 inder* recommended by Fr. E. Schulze (1)^ 

 which makes possible at the same time the 

 transfer from xylol into Canada balsam. In 

 this vessel, whose construction is evident from 

 the accompanying Fig. 7 without further ex- 

 planation, are placed three different fluids in 

 layers above one another. Below is xylol- 

 Canada balsam, next xylol, and finally alcohol. 

 In the latter are placed the previously dehy- 

 drated objects. If they are pretty small they 

 sink so gradually to the bottom that they come 

 Canada balsam without collapsing. 

 By means o f t he cock on the side of the cylin- 

 der the xylol and alcohol may be drawn off, and the prepara- 

 tions may then be removed directly to balsam on the slide. 



F c G 'iind7r Set Aftfr mto 

 / r . E. schuize. 



(c) Transfer into the Enclosing Medium. 



22. For enclosing zoological or botanical preparations in 

 Canada balsam one ordinarily uses fluid Canada balsam, 

 prepared by dissolving this resin in chloroform, xylol, or 

 some similar solvent. The solution in xylol 

 is especially to be recommended. This may 

 be poured into a wide-mouthed glass (cf. 

 Fig. 8), whose cover fits over the mouth 

 outside, and is so high that there is room 

 for a small glass rod in the closed vessel. t 



No collapse occurs in transferring an ob- 

 ject from clove-oil or xylol to this fluid Can- 

 ada balsam, as a rule. For very delicate 

 objects the ordinary xylol-balsam may well 

 be diluted with xylol, and then the latter 

 may be allowed to gradually evaporate. 



* This may be obtained of Warmbrunn, Quilitz & Co., Berlin, C., Rosen- 

 thalerstr., 40, at the price of Mk. 3.25 (80 cents). 



f Such glasses among others may be obtained at 60 pf. (15 cents) each of 

 Dr. G. Grlibler (Leipzig, Bayerische Str., 12). 



