CONTENTS. 



PART FIRST. GENERAL METHODS. 



PAGE 



T. The Observation of Living Plants and Tissues. 1-5 I 



2. The Investigation of Dried Plants. 6, 7 5 



3. Maceration. 8, 9 6 



4. Swelling. 10 ' 8 



5 Clearing. 11-27 8 



A. Chemical Clearing-methods. 12 9 



B. Physical Clearing-methods. 13-27 n 



I. The Ordinary Method of Transfer from Water to Canada 



Balsam. 14-22 12 



II. The Transfer from Water to Canada Balsam without 



Alcohol. 23-25 17 



III. The Use of other strongly refractive Mounting Media 



26, 27 . . 18 



6. Live Staining. 28 19 



7. Fixing and Staining Methods. 29-40 20 



A. Fixing. 32-34 - 21 



B. Removal of Fixing Fluids. 35 22 



C. Staining. 36-39 . . ' 24 



D. Fixing and Staining Microscopically Small Objects. 40 . . 27 



8. Microtome Technique. 41-52 , 29 



I. Imbedding in Paraffine. 43-49 31 



la. Imbedding in Celloidin. 4ga 35, 



II. The Attachment of Sections. 50-52 37 



9. Making Permanent Preparations. 53-62 40 



PART SECOND. MICROCHEMISTRY. 

 A. INORGANIC COMPOUNDS. 



1. Oxygen. 63 44 



2. Peroxide of Hydrogen. 64-67 45 



3. Sulphur. 68-70 47 



4. Hydrochloric Acid and its Salts. 71 48 



vii 



