MICROCHEMIS TR Y. 135 



cording to Loew (I), the plastin prepared macrochemically 

 byReinke is to be regarded simply as an impure albuminoid 

 preparation. This author also showed that the cytoplasm- 

 regarded by other authors as free from albumen gives the 

 protein-reaction after being first treated for a time with caustic- 

 potash (cf. 228 and Loew, II). 



d. Cytoplastin, Chloroplastin, Metaxin, Pyrenin, Amphi- 

 pyrenin, Chromatin, Limn, and Paralinin. 



238. According to the investigations of Schwarz (I), the 

 protoplasmic body is made up of eight different proteids 

 which are limited in their distribution to very special differ- 

 entiations of the protoplasm, and which should be compara- 

 tively easy to distinguish microchemically according to the 

 tables of their reactions prepared by this author. But if one 

 examines the separate results of his observations, as described 

 in detail, it is found that the most of the reagents used have 

 given very different results even with the few objects ex- 

 amined, and that the author's own observations do not at all 

 always correspond with the special statements of his tables. 

 There can no longer be any cloubt that the substances distin- 

 guished by Fr. Schwarz do not represent uniform, chemically 

 definable substances. Further studies must show whether 

 the reagents used by him are capable of rendering good 

 service in the study of the morphological elements of the 

 protoplasm. This seems most probable in case of the 

 " pretty concentrated " solution of copper sulphate used by 

 Schwarz (I, 116), which dissolves only the chromatin* in the 

 nucleus and fixes all its other constituents well. A mixture 

 of one volume of a 10$ aqueous solution of potassium ferro- 

 cyanide, two volumes of water, and half a volume of glacial 

 acetic acid also dissolves only chromatin ; but this reagent 

 is not adapted to fixing, since it causes swelling. 



239. But, since the nomenclature introduced by Fr. 



* [Malfatti (I) states that copper sulphate does not dissolve chromatin, but 

 forms an unstainable precipitate with it.] 



