222 BOTANICAL MICROTECHNIQUE. 



organism, of calcium oxalate. For their reactions 85 to- 

 88 may be consulted. It may be observed here that calcium 

 oxalate crystals can occur even in the interiors of the glo- 

 boids, as, for example, inside of the large protein-grains of 

 the seed of Vitis vinifera (cf. Fig. 50, IV, g). 



10. Protein Crystalloids. 



393. The protein crystalloids contained in the cytoplasm 

 or in the cell-sap agree essentially with the crystalloids 

 contained within the nucleus, the chromatophores, and the 

 protein-grains (cf. 343, 359, and 385). Besides very 

 regular forms, like those from the tubers of Solatium tube- 

 rosum (Fig. 38, k] or from the epidermis of the leaf of 

 Polypodium irreoides (Fig. 51, I, /), one finds also spindle- 



Fro. 51. I, epidermal cell of the lower face of the leaf of Polypodium irreoides; k, crys- 

 talloid ; z, nucleus; <r, chloroplasts. II, crystalloids from the wall of the ovary of 

 Gratiola officinalis. III. crystalloid (/fe), chloroplasts (<r), and granula (g) from a 

 spongy-parenchyma cell of Passiflora ccerule*. IV, crystalloids from the subepidermal 

 parenchyma of the leaf of Vanda furva. 



shaped or needle-like forms, or such as are variously bent 

 (Fig. 51, II-IV). Molisch (II) observed completely ring- 

 shaped crystalloids in the leaves of various species of Epi- 

 phyllum. 



For the recognition of these crystalloids the staining 

 methods detailed in 345 to 347 may best supplement the 

 study of living material. 



394. As has been recognized especially by J. Klein (I), 



