PART I 

 CULTURE METHODS AND TECHNIQUE 



CHAPTER I 



ISOLATION AND PURE-CULTURE METHODS 



LOEFFLER, FR. Vorlesungen iiber die geschichtliche Entwickelung der Lehre 

 von den Bakterien 1 : 252pp. j pis. 37 figs. 1887. Leipzig. 



SMITH, ERW. F. Bacteria in Relation to Plant Diseases. Carnegie Inst. of 

 Washington. Publication 27 (Vol. I): 285 pp. ji pis. 145 figs. 1905. 



(Text-Books and Manuals of Bacteriology.) Nearly all texts on general bac- 

 teriology devote considerable space to methods of culture work. 



FIG. i. YIKW IN LABORATORY EQUIPPED FOR PLANT PHYSIOLOGY AND 

 PATHOLOGY. (Photograph by O. Butler) 



The student who is interested in the fungous diseases of plants 

 will find it desirable at the outset to acquire a knowledge of pure- 

 culture methods. The investigator in plant pathology can only pro- 

 ceed confidently in his work when he lias had practical training in 



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