CONTENTS 



PAGE 



INTRODUCTION i 



PART I. CULTURE METHODS AND TECHNIQUE 



CHAPTER 



I. ISOLATION AND PURE-CULTURE METHODS 9 



I. The Development and Application of Culture Methods . 10 



II. Cleaning Glassware 12 



III. The Principles and Methods of Sterilization 15 



IV. Preparation of Culture Media 23 



Liquid Media 23 



Solid Media 26 



Neutralization of Culture Media 33 



V. Present Method of isolating Organisms 34 



II. TECHNIQUE OF FIXING, IMBEDDING, AND STAINING ... 41 



I. Fixing 41 



II. The Paraffin Process : Infiltration and Imbedding ... 45 



III. Staining 4 8 



PART II. PHYSIOLOGICAL RELATIONS 



III. GERMINATION STUDIES 55 



IV. GENERAL RELATIONS TO ENVIRONMENTAL FACTORS ... 62 



I. Saprophytism and Parasitism 62 



II. General Relations to Climatological Factors 66 



III. Special Relations to Environmental Factors 69 



V. ARTIFICIAL INFECTION 76 



VI. THE PRINCIPLES OF DISEASE CONTROL 85 



I. Methods of Control 85 



II. Preparation of Fungicides 88 



PART III. FUNGOUS DISEASES OF PLANTS 



VII. GENERAL CLASSIFICATION 93 



I. Fungous Diseases and Pathology 93 



II. The Classes of Fungi 94 



VIII. MYXOMYCETES. SLIME MOLDS 97 



I. Phytomyxales (Phytomyxaceae) 97 



II. Club Root of Cabbage and Other Crucifers 97 



vii 



