4 CLINICAL BACTERIOLOGY 



Platinum needles (straight 



and looped). 

 Filter-paper. 

 Glass-cutting knife. 

 Pipettes. 

 Teats (rubber). 

 Plasticine. 



Emery-paper (Hubert's 00) . 

 Cotton-wool. 

 Bottles, stock vaccines. 

 Bottle of lysol. 

 Etc. 



The Microscope. 



For bacteriological work a sound microscope is essential. 



It must have a firm, rigid stand, and an accurate fine and 

 coarse adjustment. 



It should be fitted, or be capable of being fitted, with sub- 

 stage condenser, mechanical stage, objectives f , £, and -j^-, 

 oil immersion, and two eyepieces giving a magnification 

 up to 1,000 diameters. When higher magnifications are 

 required, it is a question of employing extra eyepieces 

 which possess higher magnifications than those usually 

 supplied with an ordinary bacteriological microscope. These 

 can be obtained from any of the leading microscope-makers. 



A comparatively new and important accessory for bac- 

 teriological work is the dark-ground condenser constructed 

 for observation of living bacteria in an unstained condition. 

 Under this mode of illumination no staining is required, 

 and the bacteria are so illuminated that they appear as 

 self-luminous bodies upon a dark background. 



The microscope we possess is a Leitz, latest model, and 

 is fitted, as above described, with an additional continuous 

 safety micrometer fine adjustment, which prevents any 

 chance of damaging the objectives if accidentally over- 

 focussed and brought into contact with the specimen slide, 

 a most essential point, at least so far as beginners are con- 



