18 



CLINICAL BACTERIOLOGY AND 



into the hot medium, the tubing fixed in the mouth by the 

 teeth, the medium sucked up to the volume mark and 

 then expelled into the test-tube, and repeated until 

 all the tubes have been charged. Care must be taken not 

 to allow any of the medium to come in contact with the 

 neck of the tube, in which case the cotton plug is liable to 

 stick. Another method is to procure a glass funnel to 

 which is fixed a piece of rubber tubing. The tubing is 



Fig. 9. — Plugged and Sloped Medium Tube. 



clipped by a spring clip (clasp forceps answer the purpose 

 well), and the whole fixed upon an iron retort-stand. The 

 funnel is filled with the medium, and, if need be, kept hot 

 by a spirit flame, or a water-jacket funnel used. The tubing 

 should now be passed down the test-tube to near the 

 bottom, and the requisite quantity allowed to run in by 

 loosening the grip of the clip. Here, also, care has to be 



