VACCINE-THERAPY 39 



Staining on Tissue. — Carbol-methylene blue and cultures 

 by Gram's method. 



Microscopically the bacilli when speculating are like drum- 

 sticks, the spores being at the ends. 



Anaerobic. — No growth at 20° C. On broth or agar 

 cultivation at 37° C. in anaerobic tubes after subjecting 

 the original material to 80° C. for thirty minutes to kill 

 vegetable forms. 



(b) Gram Negative. — Bacillus (Edematis Malign!. 



Biological Characters. — 1. In broth becomes cloudy. 



2. On gelatin bright grey colonies surrounded by spread- 

 ing borders. In stab culture 1 inch below the surface white 

 side-branches form. 



3. On agar a thick network of threads form. 

 Staining. — Carbol-methylene blue. 



Microscopic al Appearances. — The bacilli are long and 

 slender and often in threads, ends rounded, and spores are 

 formed in the middle. 



Strongly anaerobic, growing well at room or incubator 

 temperature. 



Bacillus Pyocyaneus. 



Biological Characters. — 1. In broth the growth makes 

 medium cloudy. 



2. On gelatin plates flat, irregular colonies form with 

 rapid liquefaction, the whole medium becoming green. 



3. On agar the colonies form a white upper layer, and a 

 deeper layer is coloured green. 



4. On potato a greenish-yellow or brown layer forms. 

 Staining. — Carbol-methylene blue and original culture 



by Gram's method. Contrast stain neutral red. 



Microscopical Appearances. — The bacilli are often arranged 

 in pairs, and form filaments. 



Anaerobic, growing either at room or incubator tem- 

 perature. 



