VACCINE-THERAPY 95 



contrary, should bacteria infect the bruised area, pus soon 

 forms, and, partly by gravitation and partly by digestion, 

 burrows, reaching bursas and ligamentum nuchas. The 

 ligament and the bursas being lowly vascular organs, 

 the bactericidal elements going to these parts are limited. 

 This gives the bacteria a good chance to gain a footing, 

 and, when once established, pus increases, burrows down- 

 wards, causing local death to many tissue cells, and even 

 invading the osseous structures and the medullary canal 

 itself. 



The Staphylococcus aureus may be found in conjunction 

 with the albus, and sometimes a streptococcus is present. 

 Very rarely have we found a Bacillus coli. The latter's 

 presence is more commonly discovered in fistula of the 

 anus or deep-seated wounds in the region of the rectum. 

 To make an autogenous vaccine, it will be gathered, the 

 proper bacteria should be isolated and examined and cul- 

 tivated, and an appropriate vaccine made. 



A good axiom to go upon in practice is to inject one's 

 vaccines as near the seat of disease, if it be a local one, as 

 possible. And we even go farther than this by injecting 

 the vaccines into various centres round the swelling, 

 if circumscribed ones, such as poll-evil or fistula. Opsonins 

 are largely manufactured — it is believed, in muscular and 

 subcutaneous tissue. Allen has shown that, if limbs of 

 animals or men are perfused with saline solution to remove 

 all the possible blood-elements, and the muscles cooled and 

 minced, and their plasma extracted, this extract has a 

 markedly higher index than that of blood-serum towards the 

 various bacteria — proving at least muscular, subcutaneous, 

 and intramuscular tissue contains more opsonins than the 

 blood-serum itself. 



The writer always begins by injecting 500,000,000 staphy- 

 lococci at the outset, noting the symptoms, and when 

 the improvement appears to be drawing to a close, 

 follow up by giving 1,000,000,000, repeating in five to 

 seven days 2,000,000,000. 



