VACCINE-THESAFY 101 



ing to close the sinuses. A further microscopical and 

 biological examination was made, and we found a few 

 shrivelled staphylococci, but an abundant display of very 

 active B. coli. 



The initial doses in a case of this kind should be — Strepto- 

 cocci, 100,000,000; staphylococci, 500,000,000; B. coli, 

 100,000,000 : repeated in five to seven days, according to 

 conditions, in appropriately increasing doses. Of course, 

 if there is any necrosed area in the sinus, this should 

 be removed, as Nature's sloughing efforts in these cases 

 are very indolent. Here also oft - repeated antiseptic 

 dressings should be deprecated. 



Acne — Pustular Dermatitis Contagiosa. 



This condition is seldom seen in equines in this country. 

 It occurs in the form of papules or pustules, usually on the 

 parts of the body where the harness rubs. It is due to 

 a bacillus which sets up a purulent inflammation of the 

 skin, destroying the hair follicles, and may spread all over 

 the trunk by means of the harness, grooming utensils, and 

 clothing. The presence of the acne bacillus and the skin 

 lesions it sets up seem to facilitate the growth of the 

 ubiquitous staphylococcus, which invariably is found in the 

 purulent discharges, aggravating the disease and prolong- 

 ing the condition. Streptococci may also be present in the 

 pustules. The bacillus is anaerobic — at least, at the outset 

 — and is Gram-positive. 



It is small and ovoid, occurring singly or in short chains. 

 It is non-motile. In a gelatin stab culture small colonies 

 occur ; on glucose agar white colonies grow very slowly. 



In this, as in all other mixed infections, the offending 

 bacteria should be isolated, and a vaccine made from each. 



To obtain a pure culture of the acne bacillus, take a 

 scraping from the deeper parts of the sebaceous plugs, and 

 mix the material in broth, incubating anaerobically for ten 

 to fourteen days. Should staphylococci be present, by this 

 time they will be inert, while the acne bacilli will have 



