134 CLINICAL BACTERIOLOGY AND 



By so doing the virulence of the bacilli is so attenuated 

 that the vaccine so made kills mice, but not guinea-pigs. 



Vaccine No. 2 is prepared in the same manner as No. 1, 

 save that attenuation goes on for twelve days at the same 

 temperature. 



This vaccine destroys mice and guinea-pigs, but not 

 rabbits. 



For a horse or ox g- c.c. of vaccine No. 1 is first injected 

 into an animal, and after ten to twelve days the second 

 vaccine is injected — also J c.c. About three weeks after 

 the last injection the animal is proof against the disease, 

 which immunity lasts for about twelve months. 



These vaccines are not altogether free from danger in 

 practice, and it appears some animals are less resistant to 

 the virulence of the vaccine than others. The writer, seven 

 years ago, vaccinated a dairy of fifty-three cows with 

 Pasteur's vaccine, and one case nearly died after vaccine 

 No. 1. Vaccine No. 2 was not used on her. 



An intense painful swelling occurred at the seat of injec- 

 tion ; the animal was excessively lame ; her breathing was 

 hurried and laboured ; her temperature went up to 105° F. 

 Full doses of turpentine, salicylic acid and alcohol, with 

 hypodermic injections of strychnine and caffein, were 

 repeatedly administered, and she eventually recovered. 

 Since then we have used the tail as the seat of inoculation. 



Serum Treatment. 



In man several antiserums are in use : 



1. Mendez immunized horses by injecting them over a 

 long period, six to twelve months, after which he bled 

 them, and obtained the serum in the usual way. He records 

 excellent results by giving doses of 20 c.c. of this serum. 

 In cattle and sheep affected with anthrax he has also 

 obtained recoveries after using his serum. 



2. Sclaro, after a long period of experimenting, was 

 able to immunize a goat against the bacillus, and produced 

 a protective and curative serum. Later he treated asses in 



