192 



CLINICAL BACTERIOLOGY AND 



collected from the tail and throat is mixed together, a 

 preservative added of the following : 



Ac. carbolic. ... ... ... 5 - 5 c.c. 



Glycerine ... ... ... 2O0 c.c. 



Aquas dest. ... ... ... 74*5 c.c. 



100 c.c. of the above is added to 1 litre of serum, and is 

 placed in 10, 20, 30 c.c. bottles and sealed ready for use. 

 The dose for an adult pig is from 10 to 30 c.c. 



COTTON WOOC 

 TWISTED ROUND 

 THE STEM 



tube 



"1etal cisc 

 for pressing 

 serum out of 

 blooo clot 



Fig. 37. — Vessel used at the Serum Institute for collecting 

 Blood from the Tail of an Immune Pig. 



Pigs from five to ten days old receive 5 c.c. The serum 

 is injected subcutaneously behind the ear. In those 

 cases where a pig is being hyper-immunized and large 

 quantities of serum is used, one-half is injected behind one 

 ear and the other half behind the other. 



When pigs are injected with the immune serum, im- 

 munity is only conferred for a period of three weeks. This 

 period, of course, is of too short a duration to be of any 

 practical use. Should, however, the pig after injection 



