8 



APPARATUS. 



the German one of using bent glass rods for this purpose. Everyone 

 has encountered the difficulties attendant upon the bending of platinum 

 wires and also the possibility of destroying your organisms by an 

 insufficiently cooled wire. 



CLEANING GLASSWARE. 



It is a routine in our laboratory for everything to go through the 

 sterilizer at 125 C. before anything else is done. This is a safe rule 



FIG. 2. i, Inoculation of tubes; 2, plugging of tubes; 3, filling tubes; 

 4, Smith's fermentation tube; 5, Durham's fermentation tube. 



when dealing with dangerous pathogenic organisms. As soon as 

 taken out of the sterilizer the contents are emptied, and the tubes or 

 dishes placed in a i% solution of washing soda and boiled. This 

 thoroughly cleans them. As the washing soda slightly raises the 

 boiling-point and also makes the spores more penetrable, it would 

 appear that under ordinary circumstances, it would be sufficient to 

 place all contaminated articles in a dishpan with the soda solution, 



