i 1 1. 1. o.\. ig 



To summarize: 



Take Peptone, 10 grams 



Sodium chloride, 5 grams 



50% meat infusion, 1000 c.c. 



Dissolve the peptone and sodium chloride in the meat infusion and 

 add enough N/i NaOH to make the reaction +i. 



Put the solution in the inner compartment of a rice cooker and bring 

 to the boiling-point and maintain this temperature for twenty minutes. 

 The calcium chloride or sodium chloride in the outer compartment of 

 the rice cooker enables us to secure a boiling temperature for the con- 

 tents of the inner compartment. Do not stir the bouillon that is being 

 heated, as the pultaceous membranous mass of coagulated albumin 

 makes filtration easy. Filter. The filter-paper in the funnel should be 

 thoroughly wet with water before pouring on the bouillon. This is to 

 prevent clogging of the pores of the filter-paper. Make up the quantity 

 of filtrate to 1000 c.c. by adding water. 



If greater exactness is demanded than answers for ordinary 

 clinical work, it is advisable to again titrate and again adjust the 

 reaction or to simply record the exact reaction. It is more convenient 

 to have a counterpoise to balance the inner compartment and then to 

 add water to the medium until a kilo weight, in addition to the weight 

 balancing the container, is just balanced,. Then titrate, adjust the 

 reaction (if so desired) and filter. Sterilize in the autoclave at 115 

 to 120 C. for fifteen minutes or in the Arnold on three successive days. 

 The use of a balance is preferable in the preparation of bouillon, 

 necessary in making gelatin and imperative in making agar media 



BOUILLON MADE FROM LIEBIG'S MEAT EXTRACT. 



Place in a mortar 3 grams of Liebig's extract, 10 grams of pep- 

 tone and 5 grams of sodium chloride. Dissolve the whites of one or 

 two eggs in 1000 c.c. of water. Then add this egg-white water, little 

 by little, to the extract, peptone and salt in the mortar until a brownish 

 solution is obtained. Pour this into the inner compartment of a rice 

 cooker; apply heat to the outer compartment containing the salt or 

 calcium chloride solution, allow to come to a boil and to continue 



