30 STAINING METHODS. 



Stained by Gram's method. Not stained by Gram's method. 



B. aerogenes capsulatus. B. proteus. 



Odium albicans. B. of influenza. 



Mycelium of actinomyces. B. of bubonic plague. 



B. of chancroid. 



B. of Koch- Weeks. 



Gonococcus. 



Method for Staining Acid-fast Bacilli. i. Carbol fuchsin' 

 with gentle steaming for one to two minutes or in the cold three to 

 five minutes. 



2. Wash in water. 



3. Decolorize in 95% alcohol containing 3% of hydrochloric acid 

 (acid alcohol), until only a suggestion of pink remains almost white. 



4. Wash in water. 



5. Counterstain in saturated aqueous solution of methylene blue 

 or with LofBer's methylene blue. 



6. Wash, dry and mount. 



A very beautiful stain for bacteria in pus, etc., is Pappenheim's 

 solution. 



Sat. aqueous sol. methyl green 50 c.c. 

 Sat. aqueous sol. pyronin. 15 c.c. 



The bacteria are stained red; cell nuclei blue to purple. 



Smith's formol fuchsin. 



Saturated alcoholic solution basic fuchsin, 10 c.c. 

 Methyl alcohol, 10 c.c. 



Formalin, * 10 c.c. 



Distilled water to make 100 c.c. 



This gives a very sharp differentiation of bacteria and nuclear 

 structures. It has a purplish tinge. Fixation by heat gives the best 

 staining. Allow the stain to act for two to ten minutes. It should 

 not be used until after standing twenty-four hours, and after standing 

 about two weeks it appears to lose its sharp staining power. 



