CONTENTS. 



PART I. BACTERIOLOGY. 



CHAPTER I. APPARATUS. 



The Microscope, i; Apparatus for sterilization, 4; Cleaning glassware, 

 8; Concave slides, fermentation tubes. 9; Incubators, n; Bacterio- 

 logical pipettes, 12. 



CHAPTER II. CULTURE MEDIA. 



Xutrient bouillon, 15; Standardization of reaction, 17; Sugar-free 

 bouillon, 20; Glycerine bouillon, 20; Peptone solution, 21; Nutrient 

 agar, 21; Glycerine agar egg medium, 22; Gelatine, 22; Litmus 

 milk, 23; Potato, 23; Blood serum. 24; Blood agar, 24; Bile and 

 faeces media, 25. 



CHAPTER III. STAINING METHODS. 



Loffler's methylene blue, 28; Carbol fuchsin, 28; Gram's method, 28; 

 Acid-fast staining, 30; Neisser's stain, 31; Capsule staining, 31; 

 Flagella staining, 32; Spore staining, 32. 



CHAPTER IV. STUDY AND IDENTIFICATION OF BACTERIA. GENERAL CON- 

 SIDERATIONS. 

 Methods of isolating bacteria, 34; Classification, 36; Use of keys, 38. 



CHAPTER V. STUDY AND IDENTIFICATION OF BACTERIA. Cocci. 



Key, 41; Streptococci, 42; Sarcinae, 43; Staphylococci, 44; Pneumo- 

 coccus, 46; Gram negative cocci, 47. 



CHAPTER VI. STUDY AND IDENTIFICATION OF BACTERLA. SPORE-BEARING 



BACILLI. 



Key, 53; Anthrax, 54; Cultivation of anaerobes, 57; Malignant oedema, 

 59; B. botulinus, 60; B. tetani. 62; B. aerogenes capsulatus, 64. 



CHAPTER VII. STUDY AND IDENTIFICATION OF BACTERLA. BRANCHING, 



CURVING BACILLI. MYCOBACTERIA. CORNYEBACTERIA. 

 Acid-fast bacilli, 67; Tubercle bacillus, 68; Leprosy bacillus, 70; 

 Xon acid-fast branching bacilli 71; B. mallei, 72; B. diphtheriae, 73; 

 Hofman's bacillus, 76; B. xerosis, 77. 



CHAPTER VIII. STUDY AND IDENTIFICATION OF BACTERIA. 



Gram negative bacilli, Hemophilic bacteria, 78; Influenza bacillus, 

 79; Friedlander's bacillus, 81; Plague, 81; Eberth, Gartner and Esche- 

 rich groups, 85; Typhoid, 86; Dysentery 89; Chromogenic bacilli, 92. 

 ix 



