IO8 STUDY AND IDENTIFICATION OF MOULDS. 



Glycerin agar, bread paste or potato media are all suitable, but the 

 best medium is that of Sabouraud: 



Maltose, 4 grams. 



Peptone, i grams. 



Agar, 1.5 grams. 



Water, 100 c.c. 



Make the reaction about + 2. 



Before inoculating media with moulds, some recommend placing 

 the material in 60% alcohol for one or two hours to kill the bacteria. 

 The moulds withstand such treatment. 



In cultivating moulds it is best to use small Erlenmeyer flasks, 

 containing about one-fourth of an inch of media on the bottom, for 

 the development of the colonies. In order to separate the mould we 

 may take the hair or scales on a sterile slide and cut them into small 

 fragments with a sterile knife. Then moisten a platinum loop from 

 the surface of an agar slant, touch a fragment with the loop, and when 

 it adheres transfer it to the agar slant. Make four or five inoculations 

 on the surface and from suitable growth, after four to seven days, 

 inoculate the medium in the Erlenmeyer flask. 



Plauth recommends receiving the mould material between two 

 sterile glass slides. Seal the edges of the slides w ; L h wax and place 

 the preparation in a moist chamber for four to seven days. From 

 developing fungus growth inoculate the medium in the Erlenmeyer 

 flask. A Petri dish containing several layers of thoroughly moistened 

 filter-paper in top and bottom, makes a satisfactory moist chamber. 



