14 CHAPTER II. 



PREYH;R (Mitth. Zool. Stat. Neapel, Bd. vii, 1886, p. 27) re- 

 commends chloroform water for anaesthetising star-fishes. 



16. Ether and Alcohol may be administered in the same 

 way. ANDRES has obtained good results with Actiniae by the 

 use of a mixture (invented by SALVATOEE LO BIANCO) contain- 

 ing 20 parts of glycerin, 40 parts of 70 per cent, alcohol, 

 and 40 parts of sea water. This mixture should be carefully 

 poured on to the surface of the water containing the animals, 

 .and allowed to diffuse quietly through it. Several hours are 

 sometimes necessary for this. 



EISIG (Fauna u. Flora Golf. Neapel, 16, 1887, p. 239) 

 benumbs Capitellidae by putting them into a mixture of one 

 part of 70 per cent, alcohol with 9 parts of sea water, a 

 method which he recommends greatly for the study of the 

 living animals. 



17. Methyl-alcohol. CORI (Zeit. f. wiss. Mik., vi, 4, 1890, 

 p. 438) prefers methyl-alcohol to all other reagents. It has 

 the advantage of having but a slight action on albumins. 

 CORI recommends a mixture composed of 10 c.c. methyl- 

 alcohol (of 96 per cent, strength), 90 c.c. water (fresh or 

 sea water), and 0*6 grm. of sodium chloride (to be added only 

 when fresh water is taken, the addition of the salt having 

 for its object to prevent maceration). It may be well to 

 add to this mixture a very few drops of chloroform (for 

 Cristatella; Zeit.f. wiss Zool., Iv, 1893, p. 626; Zeit. f. wiss. 

 Mik., x, 4, 1893, p. 475). 



18, Hydrate of Chloral, which WMS first recommended, I 

 believe, by Foettinger (Arch, de BioL, vi, 1885, p. 115), gives 

 very good results with some subjects. Foettinger operates 

 by dropping crystals of chloral into the water containing 

 the animals. For Alcyonella he takes 25 to 80 centigrammes 

 of chloral for each hundred grammes of water. It takes 

 about three quarters of an hour to render a colony sufficiently 

 insensible to allow of fixing. Foettinger has obtained satis- 

 factory results with marine and fresh-water Bryozoa, with 

 Annelida, Mollusca, Nemertians, Actiniae, and with Asteracan- 

 thion. He did not succeed with Hydroids. 



