PREFACE. 



THE present edition is the outcome of a double revision of 

 the text of the last edition. I revised that text very thoroughly 

 in 1897 in conjunction with Prof. Paul Mayer, in view of the 

 preparation of the German edition that has since appeared.* 

 But I have not contented myself with taking the text thus 

 settled as the basis of the present edition and merely adding 

 such newer methods as have appeared up to date. 



For on the one hand the space taken up by description of 

 recent processes has turned out to be so considerable as to call 

 for a very severe condensation of the text throughout, in order 

 to keep the whole within the old limits of size limits which, 

 it seems to me, cannot be much increased without impairing 

 the handiness of the volume : so that a re-revision seemed 

 necessary on that head. And on the other hand it also 

 seemed to me desirable to amplify even more fully than was 

 done in the last edition many of the introductory portions of 

 the different chapters, and to discuss still more fully the 

 principles of some of the methods described. 



Both of these points have been carried out. Phraseology 

 has been curtailed throughout to the utmost limits consistent 

 with clearness. Rejection of superfluous methods has been 

 carried out wholesale, even to the extent of suppression of a 

 large number of the references to rejected methods that were 

 given in the last edition. The former chapter on Hardening 

 Agents has been suppressed and its contents incorporated 



* Grundzilge der mikroskopischen Technikfilr Zoologen und Anatomen, 

 von A. B. Lee und Paul Mayer; Berlin, Friedlaender und Sohn, 1898. I 

 refer to this edition under the abbreviated title of Grundzilge. 



